Manganese (Mn), one of the most abundant elements on the earth,1 is essential to human health2 and its over-exposure is considered a risk factor for neurodegenerative disorders such as Parkinson’s disease3. High Mn concentration in water and food represents contamination sources for the population.4 Among additional source is gasoline combustion that releases Mn particles within the respirable size range5 for the presence of MMT, an organic Mn-containing fuel additive. Despite evidence of structural and functional damage of the CNS induced by Mn-based compounds, behavioural effects of chronic exposure to sub-lethal MMT are still limited. We used the zebrafish (Danio rerio), a toxicology and behavioral study model,6 and the Y-Maze task7 to test the hypothesis that embryo exposure to 100 mM MMT (corresponding to 20 µM Mn2+, that reflects the exposure of individuals in highly polluted cities8) may affect fish behaviour. Results show alterations in detectable behavioral traits in larvae and cognitive impairments in exploration, orientation and spatial memory in 5 month old specimens. 1. Siegel A et al. CRC Press, 2000, p 816. 2. Aschner M et al. Toxicol Appl Pharmacol 2007, 221:131–147. 3. Schapira AH Trends Pharmacol Sci 2009, 30:41-7. 4. Oulhote Y et al. Environ Health Perspect 2014, 122:1343-50. 5. Frumkin H et al. Am J Ind Med 1997, 31:107-15. 6. Green AJ et al. Comp Biochem Physiol C Toxicol Pharmacol 2017, S1532. 7. Cognato Gde P et al. Neurobiol Learn Mem 2012, 98:231-328. 8. Joselow MM et al. Am J Public Health 1978, 68:557–560.
A behavioural zebrafish model of methylcyclopentadienyl manganese trycarbonil (MMT) environmental toxicity / Angiulli, E.; Fasano, G.; Suarez Godoy, R.; Consalvo, A.; Franco, C.; Alleva, E.; Cioni, C.; Ciavardelli, D.; Biffali, E.; Ekker, M.; Sordino, P.; Canzoniero, L. M. T.; Toni, M.. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - 62:supplement 1(2018), pp. 11-11. (Intervento presentato al convegno 64° CONVEGNO GEI SOCIETÀ ITALIANA DI BIOLOGIA DELLO SVILUPPO E DELLA CELLULA tenutosi a L’Aquila).
A behavioural zebrafish model of methylcyclopentadienyl manganese trycarbonil (MMT) environmental toxicity
E. AngiulliPrimo
;E. Alleva;C. Cioni;M. ToniUltimo
2018
Abstract
Manganese (Mn), one of the most abundant elements on the earth,1 is essential to human health2 and its over-exposure is considered a risk factor for neurodegenerative disorders such as Parkinson’s disease3. High Mn concentration in water and food represents contamination sources for the population.4 Among additional source is gasoline combustion that releases Mn particles within the respirable size range5 for the presence of MMT, an organic Mn-containing fuel additive. Despite evidence of structural and functional damage of the CNS induced by Mn-based compounds, behavioural effects of chronic exposure to sub-lethal MMT are still limited. We used the zebrafish (Danio rerio), a toxicology and behavioral study model,6 and the Y-Maze task7 to test the hypothesis that embryo exposure to 100 mM MMT (corresponding to 20 µM Mn2+, that reflects the exposure of individuals in highly polluted cities8) may affect fish behaviour. Results show alterations in detectable behavioral traits in larvae and cognitive impairments in exploration, orientation and spatial memory in 5 month old specimens. 1. Siegel A et al. CRC Press, 2000, p 816. 2. Aschner M et al. Toxicol Appl Pharmacol 2007, 221:131–147. 3. Schapira AH Trends Pharmacol Sci 2009, 30:41-7. 4. Oulhote Y et al. Environ Health Perspect 2014, 122:1343-50. 5. Frumkin H et al. Am J Ind Med 1997, 31:107-15. 6. Green AJ et al. Comp Biochem Physiol C Toxicol Pharmacol 2017, S1532. 7. Cognato Gde P et al. Neurobiol Learn Mem 2012, 98:231-328. 8. Joselow MM et al. Am J Public Health 1978, 68:557–560.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
